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42) Prague Medical Report / Vol. 111 (2010) no(prenominal) 1, p. 4254 Comparison of tierce Methods for Determination of Glucose Dohnal L.1, Kalousová M.2, Zima T.2 Reference science lab for Clinical Chemistry of the Czechoslovakian Ministry of Health, Prague, Czech Republic; 2 Charles University in Prague, First efficaciousness of Medicine and General University Hospital, launch of Clinical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic 1 Re c e i v e d J u l y 1 3 , 2 0 0 9 ; A c c e p t e d J a n u a r y 1 9 , 2 0 1 0 . tonality words: Glucose electrochemical voice Spectrophotometric termination Repeatability Reproducibility comparing Passing-Bablok regression Traceability Abstract: fail refers comparison of three manners for glucose determination precision (repeatability, reproducibility), traceability to SRM 965a NIST, comparability in kindred-pools and in patients samples: Electrochemical determination on passing GL (DiaSys, Germany) in hemolyzate GL mode, spectrophotometric determination development hexokinase (Glucose System Reagent 800, Olympus) HKL method and exploitation glucose dehydrogenase (Glucose Gluc-DH, EcolineS+, DiaSys, Germany) GDL method in hemolyzate.

For demo differences between the absorption of glucose in hemolyzed blood and corresponding blood plasma, spectrophotometric determination using hexokinase in plasma was utilise (Glucose System Reagent 800, Olympus) HKP method. Coefficients of diversity characterizing precision under repeatability and reproducibility conditions be not eminent than 3.0% for the GL method, 6.3% for the GDL method and 15.8% for the HKL method with downcast sensitivity. For glucose assiduousness less than 8 mmol/l, HKL disco biscuitds to give spurn results than GDL, and GL gos to give higher results than GDL. For glucose concentration about 2 mmol/l, the results of glucose in plasma HKP method tend to be significantly set down (by more than ten percent) than in corresponding total (hemolyzed) blood. HKL...If you neediness to get a just essay, order it on our website: Orderessay

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